Method of Sample (gDNA) Preparation for Sending out - Revision history

ZHOU YANG at 12:51, 25 November 2011

2011-11-25T12:51:24Z

ZHOU YANG at 12:48, 25 November 2011

2011-11-25T12:48:52Z

ZHOU YANG: Created page with "

1. Sample(gDNA) Preparation

1) DNA should be dissolved in TE ..."

2011-11-25T12:48:04Z

Created page with "<h1>1. Sample(gDNA) Preparation</h1> 1) DNA should be dissolved in TE ..."

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<h1>1. Sample(gDNA) Preparation</h1>
 
1)  DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.
2)  Do Not Dissolve in Water
 
 
<h1>2. Sample(gDNA) Requirement</h1>
 
1)   Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul
 This amount is required for
a.  Whole genome sequencing (30X)
b.  Whole exome sequencing (50X)
c.  Genotyping
2)  Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination
 
 
<h1>3. Sample(gDNA) Packing Information </h1>
 
This way is only available when DNA is dissolved in TE or Tris buffer.
1)  If not, please pack DNA with icepack in icebox and use Fedex or DHL service
2)  Mark sample information on tube (concentration, sample name…what you want)
3)  Seal the cap of tube with parafilm well
4)  Wrap the tube with paper towel or equivalent
5)  Put into the 50 ml Falcon tube and close the cap
6)  Send the sample with air cushion envelop by FEDEX or DHL or equivalent
 
 
<h1>4. Requirement of Sample(gDNA) Information</h1>
 
1)  Sample type: DNA
2)  Condition: DNA in TE buffer or EB buffer or equivalent. Not in water
3)  Gel image of DNA electrophoresis
4)  Species
5)  No. of Tubes
6)  Concentration (ng/μl)
7)  Volume (μl)
8)  Total Quantity (μg)
9)  OD260/280
10)  OD260/230
 
 
 
Please download and refer to the following documents for information on gDNA methods of preparation and delivery.
 
1.<a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a>
2.<a href="/userfiles/Declaration_of_Shipment_for_anyone(1).doc">Declaration of Shipment</a>

@@ Line 1: / Line 1: @@
-<h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1.&nbsp;Sample(gDNA) Preparation</span></h1>
+<h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Sample(gDNA) Preparation</span></h1>
 <p>1)&nbsp;&nbsp;DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
 <p>2)&nbsp;&nbsp;Do Not Dissolve in Water</p>
 <p>&nbsp;</p>
-<h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2.&nbsp;Sample(gDNA) Requirement</span></h1>
+<h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">Sample(gDNA) Requirement</span></h1>
 <p>1)&nbsp;&nbsp;&nbsp;Amount and concentration spec.&nbsp;: over 20 ug of gDNA with &gt;50 ng/ul</p>
 <p>&nbsp;This amount is required for</p>
@@ Line 11: / Line 11: @@
 <p>2)&nbsp;&nbsp;Sample(gDNA) should be&nbsp;: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
 <p>&nbsp;</p>
-<h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3.&nbsp;Sample(gDNA) Packing Information&nbsp;</span></h1>
+<h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">Sample(gDNA) Packing Information&nbsp;</span></h1>
 <p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
 <p>1)&nbsp;&nbsp;If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
@@ Line 20: / Line 20: @@
 <p>6)&nbsp;&nbsp;Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
 <p>&nbsp;</p>
-<h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4.&nbsp;Requirement of Sample(gDNA) Information</span></h1>
+<h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">Requirement of Sample(gDNA) Information</span></h1>
 <p>1)&nbsp; Sample type: DNA</p>
 <p>2)&nbsp; Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>

@@ Line 1: / Line 1: @@
 <h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1.&nbsp;Sample(gDNA) Preparation</span></h1>
 <p>1)&nbsp;&nbsp;DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
 <p>2)&nbsp;&nbsp;Do Not Dissolve in Water</p>
 <p>&nbsp;</p>
 <h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2.&nbsp;Sample(gDNA) Requirement</span></h1>
 <p>1)&nbsp;&nbsp;&nbsp;Amount and concentration spec.&nbsp;: over 20 ug of gDNA with &gt;50 ng/ul</p>
 <p>&nbsp;This amount is required for</p>
@@ Line 13: / Line 10: @@
 <p>c.&nbsp;&nbsp;Genotyping</p>
 <p>2)&nbsp;&nbsp;Sample(gDNA) should be&nbsp;: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
 <p>&nbsp;</p>
 <h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3.&nbsp;Sample(gDNA) Packing Information&nbsp;</span></h1>
 <p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
 <p>1)&nbsp;&nbsp;If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
@@ Line 24: / Line 19: @@
 <p>5)&nbsp;&nbsp;Put into the 50 ml Falcon tube and close the cap</p>
 <p>6)&nbsp;&nbsp;Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
 <p>&nbsp;</p>
 <h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4.&nbsp;Requirement of Sample(gDNA) Information</span></h1>
 <p>1)&nbsp; Sample type: DNA</p>
 <p>2)&nbsp; Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>
@@ Line 38: / Line 31: @@
 <p>9)&nbsp; OD260/280</p>
 <p>10)&nbsp; OD260/230</p>
 <p>&nbsp;</p>
 <p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
 <p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
 <p>2.<span style="color: #800000"><a href="/userfiles/Declaration_of_Shipment_for_anyone(1).doc">Declaration of Shipment</a><br />
 </span></p>