http://pgi.re.kr/index.php?action=history&feed=atom&title=Polony_DNA_sequencing._2006_Curr_Protoc_Mol_Biol.Polony DNA sequencing. 2006 Curr Protoc Mol Biol. - Revision history2026-05-18T09:33:27ZRevision history for this page on the wikiMediaWiki 1.31.3http://pgi.re.kr/index.php?title=Polony_DNA_sequencing._2006_Curr_Protoc_Mol_Biol.&diff=2227&oldid=prevWikiSysop at 01:24, 14 December 20082008-12-14T01:24:02Z<p></p>
<p><b>New page</b></p><div><strong>Polony DNA sequencing.</strong><br /><br />
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Porreca GJ, Shendure J, Church GM.<br /><br />
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<span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]."><a href="javascript:AL_get(this, 'jour', 'Curr Protoc Mol Biol.');">Curr Protoc Mol Biol.</a></span> 2006 Nov;Chapter 7:Unit 7.8.<br /><br />
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<p class="affiliation">Harvard Medical School, Boston, Massachusetts, USA.</p><br />
<p class="abstract">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.</p><br />
<p class="pmid">PMID: 18265387 [PubMed - indexed for MEDLINE]<br /><br />
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</p></div>WikiSysop